{"id":3894,"date":"2017-06-20T14:18:31","date_gmt":"2017-06-20T06:18:31","guid":{"rendered":"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/?p=3894"},"modified":"2017-11-29T10:37:47","modified_gmt":"2017-11-29T02:37:47","slug":"oligos-%e5%b0%88%e6%a5%ad%e5%90%88%e6%88%90%e6%9c%8d%e5%8b%99-%e5%bc%95%e5%ad%90%e3%80%81%e8%9e%a2%e5%85%89%e6%8e%a2%e9%87%9d%e3%80%81rna-%e8%88%87-ngs-oligos","status":"publish","type":"post","link":"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/oligos-%e5%b0%88%e6%a5%ad%e5%90%88%e6%88%90%e6%9c%8d%e5%8b%99-%e5%bc%95%e5%ad%90%e3%80%81%e8%9e%a2%e5%85%89%e6%8e%a2%e9%87%9d%e3%80%81rna-%e8%88%87-ngs-oligos\/","title":{"rendered":"Oligos \u5c08\u696d\u5408\u6210\u670d\u52d9"},"content":{"rendered":"<p><span style=\"font-size: 18pt;\"><strong>Oligos \u5c08\u696d\u5408\u6210\u670d\u52d9-\u5f15\u5b50\u3001\u87a2\u5149\u63a2\u91dd\u3001RNA \u8207 NGS Oligos<\/strong><\/span><\/p>\n<p>\u5728\u9019\u88e1\uff0c\u6211\u5011\u80fd\u4f9d\u64da\u60a8\u7684\u9700\u6c42\uff0c\u5408\u6210\u6240\u9700\u7684Oligos<\/p>\n<p>\u6211\u5011\u63d0\u4f9b\u5ba2\u88fd\u5316DNA Primers\u3001qPCR Probes\u3001Next-Gen Sequencing Oligos\u8207RNA Oligos\u7684\u5408\u6210\u670d\u52d9\u3002<\/p>\n<h3><strong><span style=\"font-size: 14pt;\">DNA Primers \u5f15\u5b50\u5408\u6210<\/span><\/strong><\/h3>\n<p><img loading=\"lazy\" class=\"alignnone wp-image-3909 size-full\" src=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image002-e1498099534807.gif\" alt=\"image002\" width=\"512\" height=\"412\" \/><\/p>\n<p><img loading=\"lazy\" class=\"alignnone wp-image-3910 size-full\" src=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image004-e1498098693329.gif\" alt=\"image004\" width=\"513\" height=\"344\" \/><\/p>\n<h3><strong><span style=\"font-size: 14pt;\"><span class=\"SpellE\"><span lang=\"EN-US\">qPCR<\/span><\/span><span lang=\"EN-US\"> Probes <\/span>\u63a2\u91dd\u5408\u6210<\/span><\/strong><\/h3>\n<p><img loading=\"lazy\" class=\"alignnone size-full wp-image-3911\" src=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image006.gif\" alt=\"image006\" width=\"554\" height=\"323\" \/><\/p>\n<p><img loading=\"lazy\" class=\"alignnone wp-image-3912 size-full\" src=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image008-e1498098354853.gif\" alt=\"image008\" width=\"512\" height=\"282\" \/><\/p>\n<p><strong><span style=\"font-size: 8pt;\">\u5099\u8a3b\uff1aOnyx Quencher\u2122 (OQ\u2122) &#8211; Probe-based qPCR\u7528\u7684Sigma\u2019s\u5c08\u5229\u6d88\u5149\u5291\uff0c\u53ef\u4ee5\u7528\u65bc\u6a19\u5b9a\u5728Primers \u6216Dual-Labeled Probes\u76843\u2019 \u7aef\u3002\u5df2\u8207\u591a\u7a2e\u5e38\u898b\u7684\u87a2\u5149\u67d3\u6599\u642d\u914d\u9a57\u8b49\uff0c\u4f7f\u7528\u6548\u679c\u826f\u597d\u3002<\/span><\/strong><\/p>\n<h3><span style=\"font-size: 14pt;\"><strong><span lang=\"EN-US\">Next-<span class=\"SpellE\">Gen<\/span> Sequencing <span class=\"SpellE\">Oligos<\/span> <\/span>\u6b21\u4e16\u4ee3\u5b9a\u5e8f<span class=\"SpellE\"><span lang=\"EN-US\">Oligos<\/span><\/span><\/strong><\/span><\/h3>\n<p><img loading=\"lazy\" class=\"alignnone size-full wp-image-3928\" src=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image002-2.gif\" alt=\"image002\" width=\"634\" height=\"1000\" \/><\/p>\n<ul style=\"list-style-type: square;\">\n<li><span style=\"font-size: 8pt;\"><strong>\u4ea4\u53c9\u5f71\u97ff\u98a8\u96aa\u503c<\/strong><strong>\uff0c<\/strong><strong>\u662f\u7531\u500b\u5225\u4f7f\u7528\u4e0d\u540c\u7b49\u7d1a\u7684<\/strong><strong>adapters<\/strong><strong>\uff0c<\/strong><strong>\u5728<\/strong><strong>Illumina<\/strong><strong>\u6a5f\u53f0\u57f7\u884c<\/strong><strong>Multiplex sequencing<\/strong><strong>\u800c\u5f97\u4f86\u7684<\/strong><strong>\u3002<\/strong><\/span><\/li>\n<li><span style=\"font-size: 8pt;\"><strong>\u751f\u7522\u6642\u9593<\/strong><strong>\uff0c<\/strong><strong>\u6703\u5728\u5831\u50f9\u55ae\u4e0a\u9023\u540c\u6700\u4f4e\u4fdd\u8b49\u7522\u7387\u4e00\u4f75\u63d0\u4f9b<\/strong><strong>\u3002<\/strong><\/span><\/li>\n<\/ul>\n<h3><span style=\"font-size: 14pt;\"><strong><span lang=\"EN-US\">RNA <span class=\"SpellE\">Oligos<\/span> <\/span>\u6838\u91a3\u6838\u9178\u5408\u6210<\/strong><\/span><\/h3>\n<p><img loading=\"lazy\" class=\"alignnone size-full wp-image-3927\" src=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image002-1.gif\" alt=\"image002\" width=\"518\" height=\"481\" \/><\/p>\n<hr \/>\n<p>\u56e0\u70ba\u60a8\u7684\u7814\u7a76\u5177\u6709\u524d\u77bb\u6027\uff0c\u56e0\u6b64\u6211\u5011\u63d0\u4f9b\u8d85\u904e200\u7a2e\u4ee5\u4e0a\u6838\u82f7\u9178\u4fee\u98fe\u670d\u52d9\uff0c\u5305\u542bFAM\u3001Cy3\u3001Alex Fluor\u00ae 680\u3001Amine\u3001Phosphate\u3001Thiol\u3001Biotin\u3001Digoxigenin\u3001Spacer\u30013&#8242;-dNTP\u3001Br-dNTP\u3001Acridine\u3001Cholesterol\u3001Methyl RNA\u7b49<\/p>\n<p>\u67e5\u8a62\u66f4\u591a\u76f8\u95dc\u6838\u82f7\u9178\u4fee\u98fe\u670d\u52d9<\/p>\n<ul>\n<li><span style=\"font-size: 14pt;\"><a href=\"http:\/\/www.sigmaaldrich.com\/technical-documents\/articles\/biology\/custom-dna-oligos-modifications.html#fluorescent\" target=\"_blank\"><strong>\u87a2\u5149\u9078\u9805<\/strong><\/a><\/span><\/li>\n<li><span style=\"font-size: 14pt;\"><a href=\"http:\/\/www.sigmaaldrich.com\/technical-documents\/articles\/biology\/custom-dna-oligos-modifications.html#attachment\" target=\"_blank\"><strong>\u9644\u8457\u7528\u6a19\u5b9a<\/strong><\/a><\/span><\/li>\n<li><span style=\"font-size: 14pt;\"><a href=\"http:\/\/www.sigmaaldrich.com\/technical-documents\/articles\/biology\/custom-dna-oligos-modifications.html#binding\" target=\"_blank\"><strong>\u7d50\u5408\u7528\u6a19\u5b9a<\/strong><\/a><\/span><\/li>\n<li><span style=\"font-size: 14pt;\"><strong><a href=\"http:\/\/www.sigmaaldrich.com\/technical-documents\/articles\/biology\/custom-dna-oligos-modifications.html#spacer\" target=\"_blank\">Spacer<\/a><\/strong><\/span><\/li>\n<li><span style=\"font-size: 14pt;\"><a href=\"http:\/\/www.sigmaaldrich.com\/technical-documents\/articles\/biology\/custom-dna-oligos-modifications.html#analog\" target=\"_blank\"><strong>\u6838\u82f7\u9178\u884d\u751f\u7269<\/strong><\/a><\/span><\/li>\n<li><span style=\"font-size: 14pt;\"><a href=\"http:\/\/www.sigmaaldrich.com\/technical-documents\/articles\/biology\/custom-dna-oligos-modifications.html#intercalation\" target=\"_blank\"><strong>\u5d4c\u5165\u5f0f\u6a19\u5f0f<\/strong><\/a><\/span><\/li>\n<li><span style=\"font-size: 14pt;\"><strong><a href=\"http:\/\/www.sigmaaldrich.com\/technical-documents\/articles\/biology\/custom-dna-oligos-modifications.html#antisense\" target=\"_blank\">Antisense<\/a><\/strong><\/span><\/li>\n<\/ul>\n<p><strong><span style=\"font-size: 8pt;\">\u5099\u8a3b<span lang=\"EN-US\">: <\/span>\u6a19\u6e96\u5408\u6210\u7522\u91cf\u662f\u4f9d\u64da<span lang=\"EN-US\">20<\/span>\u500b<span class=\"SpellE\"><span lang=\"EN-US\">mer<\/span><\/span>\u4ee5\u4e0a\u7684\u5e8f\u5217\u9577\u5ea6\u505a\u9810\u4f30\uff0c<span class=\"GramE\">\u82e5\u77ed\u65bc<\/span><span lang=\"EN-US\">20<\/span>\u500b<span class=\"SpellE\"><span lang=\"EN-US\">mer<\/span><\/span>\uff0c<span lang=\"EN-US\">OD<\/span>\u503c\u53ef\u80fd\u6703\u4f4e\u65bc\u4e00\u822c\u7522\u91cf\u3002<span lang=\"EN-US\">\u00b5g<\/span>\u6578\u91cf\u662f\u4f9d\u64da<span lang=\"EN-US\">30 <span class=\"SpellE\">\u03bcg<\/span>\/OD<\/span>\u8a08\u7b97\u800c\u4f86\uff0c\u82e5<span class=\"SpellE\"><span lang=\"EN-US\">\u03bcg<\/span><\/span><span lang=\"EN-US\">\/OD<\/span>\u503c\u4f4e\u65bc<span lang=\"EN-US\">30<\/span>\uff0c\u5247<span class=\"SpellE\"><span lang=\"EN-US\">\u03bcg<\/span><\/span>\u6578\u503c\u5c07\u6703\u6bd4\u4f8b\u6027\u7684\u6e1b\u5c11\u3002\u4f7f\u7528<span class=\"SpellE\"><span lang=\"EN-US\">Phosphorothioated<\/span><\/span>\u505a\u70ba\u9aa8\u67b6\u7684\u5e8f\u5217\u6838\u82f7\u9178\uff0c\u5408\u6210\u7522\u91cf\u5c07\u6703\u53ea\u6709\u6a19\u6e96\u5408\u6210\u7522\u91cf\u9810\u4f30\u503c\u7684\u4e03\u6210\u3002<span class=\"GramE\">\u82e5\u6838<\/span>\u82f7\u9178\u505a\u5176\u4ed6\u4fee\u98fe\uff0c\u5408\u6210\u7522\u91cf\u5c07\u6703\u53ea\u6709\u6a19\u6e96\u5408\u6210\u7522\u91cf\u9810\u4f30\u503c\u7684\u4e94\u6210\u3002<\/span><\/strong><\/p>\n<hr \/>\n<p>\u60a8\u7684\u6eff\u610f\u5ea6\u662f\u6211\u5011\u7684\u552f\u4e00\u8003\u91cf\uff0c\u56e0\u6b64\u6211\u5011\u91cd\u8996\u6bcf\u4e00\u500b\u751f\u7522\u4ee5\u53ca\u7d14\u5316\u6d41\u7a0b\u3002<\/p>\n<p><strong>\u5728\u9019\u88e1\uff0c\u6211\u5011\u63d0\u4f9b\u4e09\u5927\u4fdd\u8b49\uff1a<\/strong><\/p>\n<ul style=\"list-style-type: disc;\">\n<li><strong>\u4fdd\u8b49\u60a8\u7372\u5f97\u7684\u7522\u7269\uff0c\u5e8f\u5217\u8207\u6838\u82f7\u9178\u4fee\u98fe\u5b8c\u5168\u7b26\u5408\u60a8\u7684\u8981\u6c42<\/strong><\/li>\n<li><strong>\u4fdd\u8b49\u60a8\u7372\u5f97\u7684\u7522\u7269\uff0c\u9577\u5ea6\u5b8c\u6574\u4e26\u7b26\u5408\u6700\u4f4e\u4fdd\u8b49\u7522\u91cf<\/strong><\/li>\n<li><strong>\u4fdd\u8b49\u60a8\u7372\u5f97\u7684\u7522\u7269\u91cf\uff0c\u51fa\u5ee0\u524d\u5747\u7d93D.\u503c\u8a08\u7b97\uff0c\u7522\u91cf\u8aa4\u5dee\u50c5\u5728\u00b110%\u4e4b\u9593<\/strong><\/li>\n<\/ul>\n<p><strong><a href=\"http:\/\/www.sigmaaldrich.com\/technical-documents\/articles\/biology\/custom-dna-oligos-qc-qa.html\" target=\"_blank\">\u4e86\u89e3ISO 9001:2008<\/a><\/strong><\/p>\n<p><strong><a href=\"http:\/\/www.sigmaaldrich.com\/technical-documents\/articles\/biology\/dna-oligonucleotide-synthesis.html\" target=\"_blank\">\u4e86\u89e3Oligos\u5408\u7a0b\u539f\u7406<\/a><\/strong><\/p>\n<p>\u6211\u5011\u77e5\u9053\u7d14\u5316\u65b9\u5f0f\u7684\u4e0d\u540c\uff0c\u5c07\u5f71\u97ff\u8457\u60a8\u7684\u5be6\u9a57\u54c1\u8cea\uff0c\u56e0\u6b64\u6211\u5011\u63d0\u4f9b\u4e0d\u540c\u7d14\u5316\u65b9\u5f0f\uff0c\u4ee5\u7b26\u5408\u60a8\u5be6\u9a57\u6240\u9700\uff1a<\/p>\n<p><img loading=\"lazy\" class=\"alignnone wp-image-3929\" src=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image002-3.gif\" alt=\"image002\" width=\"542\" height=\"492\" \/><\/p>\n<p>\u7d14\u5316\u65b9\u5f0f\u7c21\u4ecb\uff1a<\/p>\n<ul>\n<li><strong>Desalt<\/strong><\/li>\n<\/ul>\n<p>\u5c07\u7522\u7269\u9032\u884c\u53bb\u96e2\u5b50\uff0c\u53ef\u4ee5\u5c07\u5c0f\u5206\u5b50\u6b98\u7559\u7269\u53bb\u9664\uff0c\u4e0d\u5efa\u8b70\u7528\u4f86\u7d14\u5316\u9577\u5ea6\u5927\u65bc35 bases\u7684\u7522\u7269\u3002<\/p>\n<ul>\n<li><strong>Cartridge<\/strong><\/li>\n<\/ul>\n<p><img loading=\"lazy\" class=\"alignnone size-full wp-image-3930\" src=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image001.png\" alt=\"image001\" width=\"570\" height=\"435\" srcset=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image001-120x92.png 120w, http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image001-200x153.png 200w, http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image001-300x229.png 300w, http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image001-400x305.png 400w, http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image001-500x382.png 500w, http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image001.png 570w\" sizes=\"(max-width: 570px) 100vw, 570px\" \/><\/p>\n<p>\u5229\u7528\u5b8c\u6574\u5168\u9577\u7522\u7269\u76845\uff07\u7aef\uff0c\u9084\u4fdd\u6709\u5e36\u6709\u758f\u6c34\u6027 \u00a0DMT\u00a0group\u7684\u7279\u6027\uff0c\u53ef\u4ee5\u8207\u758f\u6c34\u6027\u5c64\u6790\u7ba1\u67f1\uff0c\u6709\u6bd4\u8f03\u7dca\u5bc6\u7684\u7d50\u5408\uff1b\u800c\u4e0d\u5b8c\u6574\u7684\u7247\u6bb5 (\u6216\u7a31\u77ed\u7247\u6bb5)\uff0c\u56e0\u70ba\u6c92\u67095\uff07-DMT\uff0c\u6240\u4ee5\u6703\u5728\u89aa\u6c34\u578b\u6d17\u6db2\u6d41\u901a\u7ba1\u67f1\u6642\uff0c\u800c\u88ab\u6c96\u6d17\u6389\uff1b\u6a19\u5b9a\u87a2\u5149\u5206\u5b50\u7684\u7522\u7269\uff0c\u4e5f\u662f\u5229\u7528\u76f8\u540c\u7279\u6027\u4f86\u9032\u884c\u7d14\u5316\u3002\u4f46\u5982\u679c\u96a8\u8457\u5408\u6210\u7522\u7269\u9577\u5ea6\u7684\u589e\u52a0\uff0c\u5e36\u67095\uff07-DMT\u7684\u77ed\u7247\u6bb5\u6a5f\u7387\u4e5f\u6703\u589e\u52a0\uff0c\u9019\u6642\u5c31\u5efa\u8b70\u8981\u6539\u4ee5HPLC\u6216\u662fPAGE\u4f86\u7d14\u5316\u3002Cartridge\u7121\u6cd5\u4fdd\u8b49\u7522\u7269\u7d14\u5ea6\uff0c\u4f9d\u6211\u5011\u7684\u7d93\u9a57\u6cd5\u5247\uff0c\u4e00\u822c\u7d0465~80%\u3002<\/p>\n<ul>\n<li><strong>RP-HPLC<\/strong><\/li>\n<\/ul>\n<p>Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) \u539f\u7406\u540cCartridge\uff0c\u4e0d\u5efa\u8b70\u7528\u4f86\u7d14\u5316\u9577\u5ea6\u5927\u65bc50 bases\u7684\u7522\u7269\u3002\u4e00\u822c\u4f7f\u7528RP-HPLC\uff0c\u53ef\u4ee5\u5f97\u5230\u7d14\u5ea6\u5927\u65bc85%\u7684\u7522\u7269\u3002<\/p>\n<ul>\n<li><strong>IE-HPLC<\/strong><\/li>\n<\/ul>\n<p><img loading=\"lazy\" class=\"alignnone size-full wp-image-3931\" src=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image004.jpg\" alt=\"image004\" width=\"552\" height=\"385\" srcset=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image004-120x84.jpg 120w, http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image004-200x139.jpg 200w, http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image004-300x209.jpg 300w, http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image004-400x279.jpg 400w, http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image004-500x349.jpg 500w, http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image004.jpg 552w\" sizes=\"(max-width: 552px) 100vw, 552px\" \/><\/p>\n<p>Ion (specifically, anion)-Exchange High Performance Liquid Chromatography (IE-HPLC)\u5229\u7528\u7522\u7269\u7684\u78f7\u9178\u5206\u5b50\u6578\u91cf\u6240\u9020\u6210\u7684\u5e36\u96fb\u7a0b\u5ea6\uff0c\u4f86\u5340\u5206\u5b8c\u6574\u7247\u6bb5\u8207\u77ed\u7247\u6bb5\u3002\u5229\u7528\u9670\u96e2\u5b50\u4ea4\u63db\u7ba1\u67f1\uff0c\u4f86\u6293\u4f4f\u6240\u6709\u5408\u6210\u7247\u6bb5\uff0c\u5728\u5229\u7528\u967d\u96e2\u5b50\u6fc3\u5ea6\u7684\u6539\u8b8a\uff0c\u5148\u5c07\u77ed\u7247\u6bb5\u8d95\u51fa\u7ba1\u67f1\u5916\uff0c\u518d\u63a5\u7e8c\u5c07\u5b8c\u6574\u7247\u6bb5\u7d14\u5316\u51fa\u7ba1\u67f1\u3002\u6b64\u65b9\u6cd5\u9650\u5236\u7528\u4f86\u7d14\u5316\u9577\u5ea6\u5c0f\u65bc40 bases\u7684\u7522\u7269\uff0c\u5f88\u9069\u5408\u7528\u4f86\u7d14\u5316\u6709\u986f\u8457\u4e8c\u7d1a\u7d50\u69cb\u7684\u7522\u7269\uff0c\u4f8b\u5982\u5e8f\u5217\u51fa\u73fe\u5f88\u591aG\u6216C\u6838\u82f7\u9178\u3002<\/p>\n<ul>\n<li><strong>PAGE<\/strong><\/li>\n<\/ul>\n<p>Polyacrylamide Gel Electrophoresis (PAGE)\u5c07\u6240\u6709\u7684\u5408\u6210\u7269\u90fd\u9032\u884c\u8b8a\u6027\uff0c\u8b93\u7522\u7269\u7684\u5e36\u96fb\u6027\u662f\u8207\u5206\u5b50\u91cf\u5927\u5c0f\u5448\u6b63\u6bd4\uff0c\u4e4b\u5f8c\u518d\u5229\u7528\u96fb\u6cf3\u4f86\u9032\u884c\u7d14\u5316\u3002PAGE\u53ef\u4ee5\u7528\u4f86\u7d14\u5316\u5e8f\u5217\u9577\u5ea6\u8d85\u904e40 bases\u7684\u7522\u7269\uff0c\u4e00\u822c\u800c\u8a00\uff0c\u53ef\u4ee5\u4f7f\u7528PAGE\u7372\u5f97\u5927\u65bc95%\u7684\u7d14\u5ea6\u3002<\/p>\n<ul>\n<li><strong>Next-Gen Sequencing Oligos<\/strong><\/li>\n<\/ul>\n<p>NGSO \u63a1\u7528\u5c08\u5229\u7d14\u5316\u65b9\u5f0f\u7d14\u5316\u7522\u7269\uff0c\u4ee5\u7b26\u5408\u50cfNGS\u9019\u6a23\u6975\u9ad8\u9748\u654f\u6027\u7684\u5be6\u9a57\u6280\u8853\u8981\u6c42\uff0c\u80fd\u6709\u6548\u6e1b\u5c11\u56e0\u5e8f\u5217\u5408\u6210\u4e2d\u6240\u5c0e\u81f4\u7684\u7de8\u78bc\u932f\u8aa4\u3002<\/p>\n<p><img loading=\"lazy\" class=\"alignnone size-full wp-image-3932\" src=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/wp-content\/uploads\/2017\/06\/image006-1.gif\" alt=\"image006\" width=\"545\" height=\"421\" \/><\/p>\n<hr \/>\n<p><span style=\"font-size: 14pt;\"><strong>\u9700\u8981\u5354\u52a9\u8a2d\u8a08Primer\u8207Probe\u55ce\uff1f<\/strong><\/span><\/p>\n<p><span style=\"font-size: 14pt;\"><strong>\u8b93\u6211\u5011\u7684<a href=\"http:\/\/www.sigmaaldrich.com\/life-science\/custom-oligos\/dna-probes\/probe-design.html\" target=\"_blank\">\u7dda\u4e0a\u7cfb\u7d71<\/a>\u70ba\u60a8\u670d\u52d9<\/strong><\/span><\/p>\n<p><span style=\"font-size: 14pt;\"><strong>\u8a02\u8cfc<a href=\"mailto:mtaw_service@merckgroup.com\" target=\"_blank\">Oligos<\/a>\u8acb\u9ede\u6211<\/strong><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p> [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":3921,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[68],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v18.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Oligos \u5c08\u696d\u5408\u6210\u670d\u52d9 - \u53f0\u7063\u9ed8\u514b<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"http:\/\/13.213.236.142\/www.merck-lifescience.com.tw\/oligos-\u5c08\u696d\u5408\u6210\u670d\u52d9-\u5f15\u5b50\u3001\u87a2\u5149\u63a2\u91dd\u3001rna-\u8207-ngs-oligos\/\" \/>\n<meta property=\"og:locale\" content=\"zh_TW\" \/>\n<meta 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